29 March 2015

Patents / Patent Applications

"I do not think there is any thrill that can go through the human heart like that felt by the inventor as he sees some creation of the brain unfolding to success... such emotions make a man forget food, sleep, friends, love, everything."

Nikola Tesla

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Methods for assaying the formation and cleavage of enzymatic covalent bond

JP2001514903A

Japan

(57) Abstract: Enzyme - A non-radioactive method of detecting a substrate reaction, the method, the reaction by conjugation movement and free electrons from the tetrazolium salt and the reaction, a single coloration comprising the step of causing color or fluorescent formazan. This method is used a plurality of compounds to assay for enzyme activity.

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A method to assay enzymatic covalent bond formation and cleavage

EP1019531A4

European Patent Office

A non-radioactive method of detecting an enzyme-substrate reaction comprising coupling said reaction with a tetrazolium salt and the transfer of free electrons from the reaction to produce a single color or fluorescent formazan. The method is used for assaying a plurality of compounds for enzyme activity.

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A method to assay enzymatic covalent bond formation and cleavage

AU9023298A

Australia

C12Q1/26 Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase

G01N2333/91057 Acyltransferases other than aminoacyltransferases (general) (2.3.1) with definite EC number (2.3.1.-)

G01N2333/916 Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)

G01N2333/924 Hydrolases (3) acting on glycosyl compounds (3.2)

Y10S435/81 Packaged device or kit

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Non-radioactive method for detecting a labelled segment and a solution or composition therefor

United States 5,354,658

A non-radioactive method of detecting a ligand and antiligand complex labelled with alkaline phosphatase or a tracer having alkaline phosphatase conjugated thereto comprises reacting the complex with bromo-chloro-indolyl phosphate (BCIP), phenazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT) and allowing the reaction to proceed to produce a colored formazan or a color change...

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Enzymatic method for detecting a labelled segment and a solution or composition therefor

United States 5,670,327

A non-radioactive method of detecting the enzymes beta galactosidase or beta glucosidase directly or for the detection of a ligand and antiligand complex is provided wherein beta galactosidase or the complex labelled with beta galactosidase or a tracer having beta galactosidase conjugated thereto is reacted with 5-bromo-4-chloro-3-indolyl-B-D-galactoside and a tetrazolium salt to produce a...

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Direct chloramphenicol acetyl transferase assay

United States 6,225,074

A direct assay for chloramphenicol acetyl transferase (CAT) has been presented wherein the assay reagent comprises chloramphenicol, an acyl CoA compound, and a tetrazolium salt, and wherein the reagent does not have any added coupling redox enzymes. In one embodiment, the reagent is mixed with the test sample and the presence of CAT is detected by an optical response. In a second embodiment, the...

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STABLE REAGENT FOR FORMAZAN-BASE ASSAY

Japan 11-190740

PROBLEM TO BE SOLVED: To obtain a reagent composition for tetrazolium-base assay, etc., by compounding 5-methyl-phenazinium methyl sulfate nitric acid and a salt of a metal such as V, Mn, Co or their mixture, etc.

SOLUTION: A reagent composition useful e.g. for the assay using the reduction of a tetrazolium salt into a formazan as a detection/signalization system for the presence of a target...more

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METHODE DE DETECTION NON RADIO-ACTIVE D'UN SEGMENT MARQUE ET SOLUTION OU COMPOSITION CONNEXE

Canada 2,155,028

Un procédé non radioactif de détection d'un complexe ligand/antiligand marqué par la phosphatase alcaline ou un traceur auquel est conjuguée une phospatase alcaline, consiste à fare réagir le complexe avec du phosphate de bromo-chloro-indolyle (BCIP), du méthosulfate de phénazine (PMS) et du tétrazolium de diméthylthiazol diphényl (MTT), et à permettre à la réaction de se poursuivre pour produire...more

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NON-RADIOACTIVE METHOD FOR DETECTING A LABELLED SEGMENT AND A SOLUTION OR COMPOSITION THEREFOR

Europe PCT/US1994/001224

A non-radioactive method of detecting a ligand and antiligand complex labelled with alkaline phosphatase or a tracer having alkaline phosphatase conjugated thereto comprises reacting the complex with bromo-chloro-indolyl phosphate (BCIP), phenazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT) and allowing the reaction to proceed to produce a colored formazan or a color change...more

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METHOD FOR ATTACHING MOLECULAR PROBES TO A SOLID SUPPORT

Europe PCT/US2005/009962

Disclosed is a novel method for attaching aldehyde, ketone or carboxyamide derivatives of molecular probes to hydrazide or hydrazine treated solid supports under alkaline conditions. In contrast to chemistries commonly used to attach biologically relevant molecules to a solid support (e.g., thiol or primary amine conjugation chemistry), the hydrazone bonds disclosed here exhibit improved chemical...more

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METHOD FOR IMMOBILIZING MOLECULAR PROBES TO A SEMICONDUCTOR OXIDE SURFACE

United States PCT/US2005/037294

A method immobilizing molecules on the surface of a semiconductor oxide substrate by forming stable bonds with hydrazine bound to the surface is disclosed. Also disclosed is a FET sensor for sensing target molecules in a solution. The FET is modified with molecular probes immobilized on the sensor surface via hydrazone bonds. The immobilized molecular probes are available to bind target molecules...more

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Direct cholesterol esterase assay

United States 20010014462

A direct assay for cholesterol esterase is provided wherein the assay reagent comprises a tetrazolium salt, a cholesterol ester an exogenous electron carrier to create an assay sample. In one embodiment the reagent is mixed with a test sample and the presence of cholesterol esterase is detected by an optical response. In a second embodiment, the reagent is mixed with a test sample and the optical...more

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A METHOD TO ASSAY ENZYMATIC COVALENT BOND FORMATION AND CLEAVAGE

Europe PCT/US1998/017122

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Stable reagent for formazan-based assay

Europe EP0971037A2

This invention relates to a stable, all-in-one aqueous reagent comprising 5-methyl-phenazinium methyl sulfate (phenazine methosulfate (PMS)), nitric acid and a metal salt wherein the metal is selected from the group consisting of vanadium, manganese, cobalt, and mixtures thereof, useful in assays which use alkaline phosphatase as a reporter enzyme and a reduction of a tetrazolium salt to a...more

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HISTIDINE DERIVATIVES AND THEIR PHARMACEUTICAL USES

Europe WO/2010/115130

Disclosed herein are the compositions and methods for a compound of Formula Ia or Ib.

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Method for immobilizing molecular probes to a semiconductor oxide surface

United States 20060121501

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NON-RADIOACTIVE METHOD FOR DETECTING A LABELLED SEGMENT AND A SOLUTION OR COMPOSITION THEREFOR

Europe EP0701626A1

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NON-RADIOACTIVE METHOD FOR DETECTING A LABELLED SEGMENT AND A SOLUTION OR COMPOSITION THEREFOR

Europe EP0701626A4

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A METHOD TO ASSAY ENZYMATIC COVALENT BOND FORMATION AND CLEAVAGE

Europe EP1019531A1

A direct assay for chloramphenicol acetyl transferase (CAT) has been presented wherein the assay reagent comprises chloramphenicol, an acyl CoA compound, and a tetrazolium salt, and wherein the reagent does not have any added coupling redox enzymes. In one embodiment, the reagent is mixed with the test sample and the presence of CAT is detected by an optical response. In a second embodiment, the...more

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A METHOD TO ASSAY ENZYMATIC COVALENT BOND FORMATION AND CLEAVAGE

Europe EP1019531A4

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RAPID, NON-ISOTOPIC METHOD FOR ALKALINE PHOSPHATASE DETECTION AND A SOLUTION OR COMPOSITION THEREFOR

United States 60/221,369

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NOVEL METHOD FOR ALPHA AMINO ACID SYNTHESES AND USES THEREOF

United States 61/211,799

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Histidine Derivatives

United States 12/918,995

Disclosed herein are the compositions and methods for a compound of Formula Ia or Ib.

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HISTIDINE DERIVATIVES

United States PCT/US2010/029818

Disclosed herein are the compositions and methods for a compound of Formula Ia or Ib.

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METHOD FOR ATTACHING MOLECULAR PROBES TO A SOLID SUPPORT

Europe WO/2005/103066A1

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Stable reagent for formazan-based assay

Europe EP0971037A3

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NON-RADIOACTIVE METHOD FOR DETECTING A LABELLED SEGMENT AND A SOLUTION OR COMPOSITION THEREFOR

Europe WO/1994/017211A1

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STABLE REAGENT FOR FORMAZAN-BASED ASSAY

Europe PCT/US 98/110273

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HISTIDINE DERIVATIVES AND THEIR PHARMACEUTICAL USES

United States US2010/029818

Disclosed herein are the compositions and methods for a compound of Formula Ia or Ib.

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A METHOD TO ASSAY ENZYMATIC COVALENT BOND FORMATION AND CLEAVAGE

Europe WO/1999/009209A1

A nonradioactive method of detecting an enzymesubstrate reaction comprising coupling said reaction with a tetrazolium salt and the transfer of free electrons from the reaction to the tetrazolium salt to produce a single colored or fluorescent formazan wherein the improvement comprises assaying a plurality of compounds for enzyme activity.

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HISTIDINE DERIVATIVES

United States US 2011/0245311

Disclosed herein are the compositions and methods for a compound of Formula Ia or Ib.

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Stable reagent for formazan-base assay

Japan 2000-060599

A reagent composition useful e.g. for the assay using the reduction of a tetrazolium salt into a formazan as a detection/signalization system for the presence of a target substance in a specimen is produced by adding and mixing an aqueous solution containing 5-methyl-phenazinium methyl sulfate, nitric acid and a salt of a metal such as vanadium, manganese, cobalt or their mixture at a...more

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Thank you.

Dennis Wright

26 September 2014

Working 'twice as hard'

One of the best explanations I have found.

FYI -

From: http://www.answers.com/Q/What_does_it_mean_if_your_IQ_is_142



				What does it mean if your IQ is 142 An IQ of 142 is classified as 'very superior intelligence.'It means that you have the potential to be capable of processing significant amounts of.

				View on www.answers.com	Preview by Yahoo

An IQ of 142 is classified as 'very superior intelligence.'

It means that you have the potential to be capable of processing significant amounts of information faster and more accurately than 95% of the people that you will come in contact with in your lifetime.

Please notice the word 'potential'. Having a high IQ does not mean that you are guaranteed success. Many people with high IQ's struggle with life because they process things differently than most people and therefor have trouble relating to those around them.

One of the best examples I have heard regarding this type of situation was from a professor of mine at Colorado State. She stated that someone with a high IQ must work twice as hard as a person with an "average" IQ. Why? Those with higher IQ's grasp concepts that normal people can't comprehend. Therefor, we must work twice as hard to find a way to explain what we have grasped in a way others can understand. Throw a learning difference like ADD/HD in the mix and you have someone that not only can process information at a ridiculous rate, but can't explain how he/she got to the conclusion.

Another problem with having a high IQ at a young age is that curriculum is often designed to target those closer to the mean IQ range of 100. High IQ individuals are able to "skate" by with minimal effort which does them no good in life after school. A high IQ without a proper education is like a modern supercomputer running Microsoft Windows 3.1. Massive computational power, small knowledge database.

Talent does what it can; Genius does what it must.
Robert Bulwer-Lytton

07 December 2013

For myself I am an optimist -

Received earlier:

" Hello Dr. Wright, my name is Dave i have a 7yr old friend, his name is Gage Driver. He is suffering from a tumor they call it DIPG he's currently going to Gillett Childrens hospital in Minneapolis, with not much success. I know Chemo/Radiation along with avastin and decadron on a 7yr old isn't the answer. Please share with me what you would do, because it's only a matter of time before my friend starts that all too unpopular tailspin, that has only one ending. I've read how you claim to have a cancer stopper but why aren't people paying attention? I myself have just returned from Mexico where stemcells have lengthened my life, I suffer from a condition called alpha one anitripsin defiecency, This procedure isn't legal in the states but yet yours is, Why sir are you sitting around? My 7yr old friend wants to be president,,,now you don't want to obstruct this countries eminent future,do you. I'm hoping you can find a way to tell me one good reason you can't help us out. Your friends Gage and Dave "

My reply:

"Dear Dave and Gage,

Thank you. Your message is understood. I ask you to understand that we are in the process of getting this new chemical entity approved for use world-wide... and yes, there is a procedure which must be followed:

FDA approval (link updated 18 March 2013)

I have been and continue to advance the 'state-of-the-art'. With sufficient funding, this new class of alpha amino acids can be in human clinical trials as soon as Summer 2012. This is all about timing and finances. Yes, many lives will be saved and many more will be spared the consequences of a cancer diagnosis.

Others are also waiting:

I heard about your research from my cousin who is a nurse. I had no idea I would ever possibly be talking to you. The word "on the street" is that you are using amino acids to cure cancer but that your clinical trials won't be starting until next year. Unfortunately I don't have that long to wait.

I had a tumor on my L4 and 11 lesions on my liver as of Sept 8. I had 10 treatments of radiation on my back and that tumor is dead. I then had 3 courses of chemo, Gemzarine and Taxotere which did absolutely nothing for my liver, I now have numerous lesions so this is a fairly aggressive cancer.

I am very interested if you think you can help me, I was extremely healthy until this hit. I didn't even have any symptoms from the liver. If it hadn't have been for the tumor on my back, I wouldn't even know there was a problem.

Yes, Dave and Gage, this does work. This compound , (S)-2-Amino-3-(3-phenylacetylimidazol-4-yl)propanoic acid, kills cancer cells - with no demonstrated toxicity to healthy cells. No surgical excision, no chemotherapy, no radiation therapy.
The only impediment to advancing the approval process is funding. I have been doing this for years - with my own personal funds and they are almost exhausted. As soon as funds are available this compound will go to GMP synthesis, CRO toxicology... and human clinical trials (Phase I - Phase I/II hybrid) can start as soon as six months afterward. This compound is eligible for expedited approval, compassionate usage exemption, accelerated review, orphan drug status. Once again, we need finances to advance the process.http://cancercure-d.blogspot.com/

http://www.freepatentsonline.com/WO2010115130A1.pdf

Thank you, again. Keep me apprised of events with yourself and Gage.

Sincerely,

Dennis Wright "

FYI - Gage died April 2012 from DIPG

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Published - with permission:

"If I do get to the end of the next part - which I intend to be the last in that series - I won't mind if you reprint it as will be, or in some form modified in a way that's mutually agreeable. I am certainly all for treatments that don't have the toxicity of chemotherapy or the dangers of radiotherapy, but I don't want to support an approach that suggests that a cancer cure may be just round the corner. I don't believe that for a moment. Naturally anything that advances cancer treatment, palliative or curative, I could hardly object to.

With best wishes,

Denis."
--
http://deniswright.blogspot.com/

Reality bites 5b: the sharp edge bluntly

home | WHAT'S NEW! | stories from my past

[to finish the story....maybe.]

How does that fit in with my chess game? In chess, as the end of the game itself approaches, the losing player's king is placed in check, and the final trap is sprung when the king is checked and has nowhere to go. That's it. Checkmate.

There is one slim hope for the losing player, and that is a stalemate, but I won't try to explain that here. Well, I did explain it, but it got too complicated and didn't add anything to this story, so a pointless paragraph is gone, and we're all the better for that.

In a stalemate, no-one wins. It's a Get Out of Jail Free card for the player who was going to lose. (Ah, sorry – I've just Monopolised my chess game....)

In my game, there will be no stalemate. Mr C won't fall for it. I may evade his attack for as long as I can, but a stalemate won't happen. I know this because of the signs that are constantly increasing in number. The return of seizures, the headaches, loss of balance and increased difficulty in walking and swallowing, the strange, apparently random tremors in other parts of my body when I'm sitting or lying down; the increasing failure to remember something that was in my mind a minute ago, whether a word or an idea. Some of these symptoms of accelerated tumour activity are not completely new, but the permutations and combinations tells their own story.

It seems the king is rapidly getting boxed in.

♖ ♖ ♖ ♖ ♖

In a real game, the losing player will see defeat coming, shake hands with the opponent, and resign the game before having to play it out to an inevitable and perhaps humiliating conclusion.

Mr C doesn't like that ending. He may play a mean game of chess, but he only seeks growth at the expense of dependency, and the great irony is that his win is his own death. He will refuse to accept the resignation of his host and he will demand that the game be played out to the bitter end.

It may well be that he's more subtle than a mere biological cell-cloning program, and is capable of tiny mutations that render yesterday's treatments ineffective, or less effective than they were. His only intelligence is to find ways past the barriers that contain him and his influence. Don't be fooled; he may well be better at that game than many give him credit for, and this means researchers can be trapped in relying on outdated remedies or approaches and faith in faulty data. But that too is another story and takes me away from this one.

Here's the blunt bit. There is no honorable resignation for me. Our society, for all its multiplicity of reasons, some logical and some idiotic, decrees that the game must be played out to the last gasp. It allows no right for the player to decide just when the game should end, and thus, on grounds of higher purpose, denies the last shred of dignity in the process. And this is specially true in the sequence of events in dying from brain cancer, or other neurological calamities for the organism, where the invasion is into the core and very centre of our being.We are no longer who we were.

I've always accepted that life, by its very nature, is not fair. I go along with that. In the natural world, fairness is not an issue; for humans, fairness is a rather simplistic idea constructed by the mind, and exists only there. If you believe in fairness or unfairness in such cases, then you have the sticky question of explaining why it happened, morally – and most of the answers I've seen to that question are far from convincing. In fact, I'll go so far as to say they usually insult my intelligence.

So to me there is a terrible cruelty, with no redeeming feature, in cloaking the right to a dignified ending to the game in platitudes, specious arguments and blind dogma. None, including bishops and those new knights of the realm, our politicians, have any right to impose this nonsense upon those who do not accept their views. They play their games with our lives; but not content with that, with our deaths as well.

This didn't end up quite as you expected, did it? Me neither."

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Saturday 7 December 2013

It has been a very hard, but special day.

Today I said goodbye to my beloved Denis.

He died at 5.10pm tonight. Peacefully, without fanfare, just as he lived.

They say that hearing is the last thing to go.

So loved by so many people. I reminded him of that near the end.

I sang him a lullaby, kissed his cheek and told him "Off you go now". He did.

He was so happy and ready for it to be over.

I am privileged to have been the one there holding his hand at the end.

The last messages I read out to him were from his darling daughters. He asked me to share them with you here.

Tracey

A Goodbye Letter

Dear Dad,

I don’t really know how to write this – I’ve never felt more clumsy with words. But I know I’m luckier than most because we’ve had so much lovely time together and I get a chance to say Goodbye and so many daughters don’t get this chance.

I guess I just want you to know I love you and feel so incredibly fortunate to have been brought up by such a wonderful dad. You taught me to strive to be caring, compassionate, and strong and purposeful. I say this because I want you to know that you don’t have to worry about me – I feel positive and inspired by the beautiful things in life and I will take good care of myself and stay true to my heart. I will always look out for Alice and we’ll stay strong together through whatever life throws our way – sisters united, no matter what.

Thank you for all your words on the blog – there are so many lessons and memories recorded there that I know I will read and reread whenever I need advice, to be cheered up, and to feel close to you.

I know you love me and are proud of me. I’ve never wanted for anything from you. You have been the most wonderful father a daughter could ever dream of.

I wish you sweet dreams, Daddy, forever your Little Girl.

Goodbye, Daddyo. I love you.

Sylvia

Haiku by Alice

Some Moments in Time

Floating through my memory

I create for you

Hockey Sticks and Balls
Daddy taught me everything
Goals I have many...

Spelling Must Be Right
Even on a shopping list
CusTURD was the best! (that was Sylvia not me actually!)

Dinosaur Project
Dad's help and coloured paper
Came top of the class.

Warm Sand, Crashing Waves

Shiny shells and Cuttlefish

Christmas by the sea

Hit the fence for four
No this wasn't the ashes
French Cricket, Dad rules!

I was only five
Numbers crunching, brain hurting
Pontoon twenty one!

Midnight wake me up!
Moon is out, the tide is right
Fishing with my Dad

Spiders on the floor
Out of the wood, hairy legs
Daddy put them out!

Favourite moments
Running, laughing, stories and fun
Daddy is the word

A pillar of strength
From this life to the next one
You will always be

xoxoxoxoxoxoxoxoxooxoxoxoxoxoxoxoxoxoxoxoxooxooxooxoxoxox

For myself I am an optimist - it does not seem to be much use being anything else.: Sir Winston Churchill

13 April 2013

Synthesis: (S)-2-amino-3-(3-phenylacetylimidazol-4-yl)propanoic acid

Objective:

Synthesis of 2-Amino-3-(3-phenylacetylimidazol-4-yl)propanoic acid.

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Materials:

Laminar flow chemical hood
Magnetic stirrer/hot plate
L-Histidine (Sigma )

Phenylacetyl chloride (Aldrich )
1N NaOH
Whatman #2 filter paper
dH20
Thermometer
400ml beaker
1000ml Ehrlenmeyer filter flask
Buchner funnel
Evaporation plates (2)

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Procedure:

47.32g His

~48 ml PAC

10:00am - 11:30am

Rx in 400ml beaker w/stir bar

Temp. = 50*-65*C

7.5ml (500ul aliquots)1N NaOH added to Rx at 11:30am

50ml H2O added at 11:35

3.25ml 1N NaOH added at 11:45am

Temp. = 55*C at 11:55

Stop Rx at 12:00

Vacuum filter (#2 Whatman paper in Buchner funnel) into 1000ml Ehrlenmeyer filter flask

Pour eluant into evaporation plates (2)

Rinse flask w/ H2O- pour into evaporation plates.

Evaporate to dryness in laminar flow hood.

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HPLC Q.C. check:

C-18 column

Mobile phase: 80 parts H2O, 20 parts CH3OH, 1 part CH3COOH

flow rate: 1ml/min

qualitative analysis performed on three (3) aliquots:

1.) Histidine control

2.) Rx beaker - after 1 hr Rx

3.) Filtered eluant (product)

Histidine control graph shows no product peaks or phenylacetic acid peak.

Rx aliquot has unreacted histidine peak, 2-amino-3-(3-phenylacetylimidazol-4-yl)propanoic acid peak, 2-amino-3-(1-phenylacetylimidazol-4-yl)propanoic acid peak, phenylacetyl linkage on primary amine of histidine (phenylacetyl - histidine) peak, phenylacetic acid peak.

Filtered eluant (product) aliquot has only product peak - 2-amino-3-(3-phenylacetylimidazol-4-yl)propanoic acid + very small (<5%) isomer peak (2-amino-3-(1-phenylacetylimidazol-4-yl)propanoic acid)
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Thank you.

Regards,

Dennis Wright
drwright442000@yahoo.com

18 March 2013

FDA Drug Approval Process

If the FDA gives the green light, the "investigative" drug will then enter three phases of clinical trials:

Phase 1 uses 20-80 healthy volunteers to establish a drug's safety and profile. (about 1 year)
Phase 2 employs 100-300 patient volunteers to assess the drug's effectiveness. (about 2 years)
Phase 3 involves 1000-3000 patients in clinics and hospitals who are monitored carefully to determine effectiveness and identify adverse reactions. (about 3 years)

The company then submits an application (usually about 100,000 pages) to the FDA for approval, a process that can take up to two and a half years. After final approval, the drug becomes available for physicians to prescribe. At this stage, the drug company will continue to report cases of adverse reactions and other clinical data to the FDA.
The research-based pharmaceutical industry currently invests some US$12.6 billion a year in new drug development. Historically, the drug development figure doubles every five years.

See also: How Drugs are Developed and Approved

15 June 2012

Proofs

Introduction to Proofs

Proofs are the heart of mathematics. Mathematics is a prerequisite for science… and understanding science is necessary for life.

If you are a math major, then you must come to terms with proofs--you must be able to read, understand and write them. What is the secret? What magic do you need to know?

There is no secret, no mystery, no magic. All that is needed is some common sense and a basic understanding of a few trusted and easy to understand techniques.

The Structure of a Proof

The basic structure of a proof is easy: it is just a series of statements, each one being either

An assumption or
A conclusion, clearly following from an assumption or previously proved result.

Directly and inevitably to the desired conclusion- without any distractions about irrelevant details. Each step should be clear or at least clearly justified. A good proof is easy to follow. And that is all.

Biological effects of S-2-Amino-3-(3-phenylacetylimidazol-4-yl) propanoic acid:

HEP G2 cell viability test -

DMEM	0	1	5	10	25	50	[mM]

0.215	1.311	1.275	1.16	0.864	0.247	0.187	#1
0.21	1.333	1.403	1.049	0.874	0.251	0.185	#2
0.221	1.274	1.386	1.126	0.842	0.271	0.185	#3
	1.264	1.332	1.042	0.797	0.253	0.189	#4

0.2153	1.2955	1.349	1.09425	0.84425	0.2555	0.1865	average
0.005508	0.032151	0.057879	0.058048	0.034219	0.01063	0.001915	Std.Dev

Additional detail: each well contains 200ul. HEP G2 is the ATCC 'gold standard' for a hepatocellular carcinoma (liver cancer) cell line. Concentrations are expressed in millimolars [0mM], [1mM], [5mM], [10mM], [25mM], [50mM]. Column labelled 'DMEM' is only growth media - with no cells. The test wells all have 2,500cells per well. Microtitre plate was incubated for 3 days at 37*Celsius. Color developed with MTS (a tetrazolium salt)-commonly used for cell viability determinations. The plate was read on a microtitre plate reader at a wavelength of 492nm. Resulting absorbances indicate complete (100%) inhibition of HEP G2 at a concentration of [25mM] (S)-2-Amino-3-(3-phenylacetylimadazol-4-yl)propanoic acid.

Rapid inhibition of difficult to treat cancer cell lines.

Independently confirmed, reproducible, effective and undisputed...

quod erat demonstrandum

And that is all.

29 March 2015

United States 5,354,658

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United States 5,670,327

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United States 6,225,074

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Japan 11-190740

PROBLEM TO BE SOLVED: To obtain a reagent composition for tetrazolium-base assay, etc., by compounding 5-methyl-phenazinium methyl sulfate nitric acid and a salt of a metal such as V, Mn, Co or their mixture, etc.

SOLUTION: A reagent composition useful e.g. for the assay using the reduction of a tetrazolium salt into a formazan as a detection/signalization system for the presence of a target...more

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Canada 2,155,028

Europe PCT/US1994/001224

Europe PCT/US2005/009962

United States PCT/US2005/037294

United States 20010014462

Europe PCT/US1998/017122

Europe EP0971037A2

Europe WO/2010/115130

United States 20060121501

Europe EP0701626A1

Europe EP0701626A4

Europe EP1019531A1

Europe EP1019531A4

RAPID, NON-ISOTOPIC METHOD FOR ALKALINE PHOSPHATASE DETECTION AND A SOLUTION OR COMPOSITION THEREFOR

United States 60/221,369

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United States 61/211,799

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United States 12/918,995

United States PCT/US2010/029818

Europe WO/2005/103066A1

Europe EP0971037A3

Europe WO/1994/017211A1

Europe PCT/US 98/110273

United States US2010/029818

Disclosed herein are the compositions and methods for a compound of Formula Ia or Ib.

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Europe WO/1999/009209A1

United States US 2011/0245311

Japan 2000-060599

Thank you.

Dennis Wright

26 September 2014

07 December 2013

Reality bites 5b: the sharp edge bluntly

A Goodbye Letter

Haiku by Alice

13 April 2013

18 March 2013

15 June 2012

The Structure of a Proof

Followers