Sunday, March 29, 2015

Patents / Patent Applications

"I do not think there is any thrill that can go through the human heart like that felt by the inventor as he sees some creation of the brain unfolding to success... such emotions make a man forget food, sleep, friends, love, everything."

Nikola Tesla


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Non-radioactive method for detecting a labelled segment and a solution or composition therefor

United States 5,354,658 
A non-radioactive method of detecting a ligand and antiligand complex labelled with alkaline phosphatase or a tracer having alkaline phosphatase conjugated thereto comprises reacting the complex with bromo-chloro-indolyl phosphate (BCIP), phenazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT) and allowing the reaction to proceed to produce a colored formazan or a color change...
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Enzymatic method for detecting a labelled segment and a solution or composition therefor

United States 5,670,327 
A non-radioactive method of detecting the enzymes beta galactosidase or beta glucosidase directly or for the detection of a ligand and antiligand complex is provided wherein beta galactosidase or the complex labelled with beta galactosidase or a tracer having beta galactosidase conjugated thereto is reacted with 5-bromo-4-chloro-3-indolyl-B-D-galactoside and a tetrazolium salt to produce a...
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Direct chloramphenicol acetyl transferase assay

United States 6,225,074 
A direct assay for chloramphenicol acetyl transferase (CAT) has been presented wherein the assay reagent comprises chloramphenicol, an acyl CoA compound, and a tetrazolium salt, and wherein the reagent does not have any added coupling redox enzymes. In one embodiment, the reagent is mixed with the test sample and the presence of CAT is detected by an optical response. In a second embodiment, the...
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STABLE REAGENT FOR FORMAZAN-BASE ASSAY

Japan 11-190740 
PROBLEM TO BE SOLVED: To obtain a reagent composition for tetrazolium-base assay, etc., by compounding 5-methyl-phenazinium methyl sulfate nitric acid and a salt of a metal such as V, Mn, Co or their mixture, etc.
SOLUTION: A reagent composition useful e.g. for the assay using the reduction of a tetrazolium salt into a formazan as a detection/signalization system for the presence of a target...more 
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METHODE DE DETECTION NON RADIO-ACTIVE D'UN SEGMENT MARQUE ET SOLUTION OU COMPOSITION CONNEXE

Canada 2,155,028 
Un procédé non radioactif de détection d'un complexe ligand/antiligand marqué par la phosphatase alcaline ou un traceur auquel est conjuguée une phospatase alcaline, consiste à fare réagir le complexe avec du phosphate de bromo-chloro-indolyle (BCIP), du méthosulfate de phénazine (PMS) et du tétrazolium de diméthylthiazol diphényl (MTT), et à permettre à la réaction de se poursuivre pour produire...more
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NON-RADIOACTIVE METHOD FOR DETECTING A LABELLED SEGMENT AND A SOLUTION OR COMPOSITION THEREFOR

Europe PCT/US1994/001224 
A non-radioactive method of detecting a ligand and antiligand complex labelled with alkaline phosphatase or a tracer having alkaline phosphatase conjugated thereto comprises reacting the complex with bromo-chloro-indolyl phosphate (BCIP), phenazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT) and allowing the reaction to proceed to produce a colored formazan or a color change...more
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METHOD FOR ATTACHING MOLECULAR PROBES TO A SOLID SUPPORT

Europe PCT/US2005/009962 
Disclosed is a novel method for attaching aldehyde, ketone or carboxyamide derivatives of molecular probes to hydrazide or hydrazine treated solid supports under alkaline conditions. In contrast to chemistries commonly used to attach biologically relevant molecules to a solid support (e.g., thiol or primary amine conjugation chemistry), the hydrazone bonds disclosed here exhibit improved chemical...more
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METHOD FOR IMMOBILIZING MOLECULAR PROBES TO A SEMICONDUCTOR OXIDE SURFACE

United States PCT/US2005/037294 
A method immobilizing molecules on the surface of a semiconductor oxide substrate by forming stable bonds with hydrazine bound to the surface is disclosed. Also disclosed is a FET sensor for sensing target molecules in a solution. The FET is modified with molecular probes immobilized on the sensor surface via hydrazone bonds. The immobilized molecular probes are available to bind target molecules...more
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Direct cholesterol esterase assay

United States 20010014462 
A direct assay for cholesterol esterase is provided wherein the assay reagent comprises a tetrazolium salt, a cholesterol ester an exogenous electron carrier to create an assay sample. In one embodiment the reagent is mixed with a test sample and the presence of cholesterol esterase is detected by an optical response. In a second embodiment, the reagent is mixed with a test sample and the optical...more
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A METHOD TO ASSAY ENZYMATIC COVALENT BOND FORMATION AND CLEAVAGE

Europe PCT/US1998/017122 
A non-radioactive method of detecting an enzyme-substrate reaction comprising coupling said reaction with a tetrazolium salt and the transfer of free electrons from the reaction to produce a single color or fluorescent formazan. The method is used for assaying a plurality of compounds for enzyme activity. 
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Stable reagent for formazan-based assay

Europe EP0971037A2 
This invention relates to a stable, all-in-one aqueous reagent comprising 5-methyl-phenazinium methyl sulfate (phenazine methosulfate (PMS)), nitric acid and a metal salt wherein the metal is selected from the group consisting of vanadium, manganese, cobalt, and mixtures thereof, useful in assays which use alkaline phosphatase as a reporter enzyme and a reduction of a tetrazolium salt to a...more
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HISTIDINE DERIVATIVES AND THEIR PHARMACEUTICAL USES

Europe WO/2010/115130 
Disclosed herein are the compositions and methods for a compound of Formula Ia or Ib. 
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Method for immobilizing molecular probes to a semiconductor oxide surface

United States 20060121501 
A method immobilizing molecules on the surface of a semiconductor oxide substrate by forming stable bonds with hydrazine bound to the surface is disclosed. Also disclosed is a FET sensor for sensing target molecules in a solution. The FET is modified with molecular probes immobilized on the sensor surface via hydrazone bonds. The immobilized molecular probes are available to bind target molecules...more
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NON-RADIOACTIVE METHOD FOR DETECTING A LABELLED SEGMENT AND A SOLUTION OR COMPOSITION THEREFOR

Europe EP0701626A1 
A non-radioactive method of detecting a ligand and antiligand complex labelled with alkaline phosphatase or a tracer having alkaline phosphatase conjugated thereto comprises reacting the complex with bromo-chloro-indolyl phosphate (BCIP), phenazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT) and allowing the reaction to proceed to produce a colored formazan or a color change...more
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NON-RADIOACTIVE METHOD FOR DETECTING A LABELLED SEGMENT AND A SOLUTION OR COMPOSITION THEREFOR

Europe EP0701626A4 
A non-radioactive method of detecting a ligand and antiligand complex labelled with alkaline phosphatase or a tracer having alkaline phosphatase conjugated thereto comprises reacting the complex with bromo-chloro-indolyl phosphate (BCIP), phenazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT) and allowing the reaction to proceed to produce a colored formazan or a color change...more
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A METHOD TO ASSAY ENZYMATIC COVALENT BOND FORMATION AND CLEAVAGE

Europe EP1019531A1 
A direct assay for chloramphenicol acetyl transferase (CAT) has been presented wherein the assay reagent comprises chloramphenicol, an acyl CoA compound, and a tetrazolium salt, and wherein the reagent does not have any added coupling redox enzymes. In one embodiment, the reagent is mixed with the test sample and the presence of CAT is detected by an optical response. In a second embodiment, the...more
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A METHOD TO ASSAY ENZYMATIC COVALENT BOND FORMATION AND CLEAVAGE

Europe EP1019531A4 
A direct assay for chloramphenicol acetyl transferase (CAT) has been presented wherein the assay reagent comprises chloramphenicol, an acyl CoA compound, and a tetrazolium salt, and wherein the reagent does not have any added coupling redox enzymes. In one embodiment, the reagent is mixed with the test sample and the presence of CAT is detected by an optical response. In a second embodiment, the...more
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RAPID, NON-ISOTOPIC METHOD FOR ALKALINE PHOSPHATASE DETECTION AND A SOLUTION OR COMPOSITION THEREFOR

United States 60/221,369 
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NOVEL METHOD FOR ALPHA AMINO ACID SYNTHESES AND USES THEREOF

United States 61/211,799 
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Histidine Derivatives

United States 12/918,995 
Disclosed herein are the compositions and methods for a compound of Formula Ia or Ib.
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HISTIDINE DERIVATIVES

United States PCT/US2010/029818 
Disclosed herein are the compositions and methods for a compound of Formula Ia or Ib.
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METHOD FOR ATTACHING MOLECULAR PROBES TO A SOLID SUPPORT

Europe WO/2005/103066A1 
Disclosed is a novel method for attaching aldehyde, ketone or carboxyamide derivatives of molecular probes to hydrazide or hydrazine treated solid supports under alkaline conditions. In contrast to chemistries commonly used to attach biologically relevant molecules to a solid support (e.g., thiol or primary amine conjugation chemistry), the hydrazone bonds disclosed here exhibit improved chemical...more
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Stable reagent for formazan-based assay

Europe EP0971037A3 
This invention relates to a stable, all-in-one aqueous reagent comprising 5-methyl-phenazinium methyl sulfate (phenazine methosulfate (PMS)), nitric acid and a metal salt wherein the metal is selected from the group consisting of vanadium, manganese, cobalt, and mixtures thereof, useful in assays which use alkaline phosphatase as a reporter enzyme and a reduction of a tetrazolium salt to a...more
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NON-RADIOACTIVE METHOD FOR DETECTING A LABELLED SEGMENT AND A SOLUTION OR COMPOSITION THEREFOR

Europe WO/1994/017211A1 
A non-radioactive method of detecting a ligand and antiligand complex labelled with alkaline phosphatase or a tracer having alkaline phosphatase conjugated thereto comprises reacting the complex with bromo-chloro-indolyl phosphate (BCIP), phenazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT) and allowing the reaction to proceed to produce a colored formazan or a color change...more
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STABLE REAGENT FOR FORMAZAN-BASED ASSAY

Europe PCT/US 98/110273 
This invention relates to a stable, all-in-one aqueous reagent comprising 5-methyl-phenazinium methyl sulfate (phenazine methosulfate (PMS)), nitric acid and a metal salt wherein the metal is selected from the group consisting of vanadium, manganese, cobalt, and mixtures thereof, useful in assays which use alkaline phosphatase as a reporter enzyme and a reduction of a tetrazolium salt to a...more
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HISTIDINE DERIVATIVES AND THEIR PHARMACEUTICAL USES

United States US2010/029818 
Disclosed herein are the compositions and methods for a compound of Formula Ia or Ib.
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A METHOD TO ASSAY ENZYMATIC COVALENT BOND FORMATION AND CLEAVAGE

Europe WO/1999/009209A1 
A nonradioactive method of detecting an enzymesubstrate reaction comprising coupling said reaction with a tetrazolium salt and the transfer of free electrons from the reaction to the tetrazolium salt to produce a single colored or fluorescent formazan wherein the improvement comprises assaying a plurality of compounds for enzyme activity. 
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HISTIDINE DERIVATIVES

United States US 2011/0245311 
Disclosed herein are the compositions and methods for a compound of Formula Ia or Ib. 
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Stable reagent for formazan-base assay

Japan 2000-060599 
A reagent composition useful e.g. for the assay using the reduction of a tetrazolium salt into a formazan as a detection/signalization system for the presence of a target substance in a specimen is produced by adding and mixing an aqueous solution containing 5-methyl-phenazinium methyl sulfate, nitric acid and a salt of a metal such as vanadium, manganese, cobalt or their mixture at a...more
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  Thank you.

Dennis Wright